(10?ml) each of buffers B, C (buffer A containing 0
(10?ml) each of buffers B, C (buffer A containing 0.2% digitonin) and D (buffer A containing 0.2% Triton X-100), and finally with 8 vol. following. First, homogenates from COS-7 cells transfected with mouse cDNA exhibited C-4-hydroxylase activity in an assay with mRNA content was regulated in a tissue-specific manner and was high in the mouse … Read more