The final His6-REX-TolA-AVI fusion proteins were produced as biotinylated proteins in the BL21 (DE3) BirA strain expressing biotin ligase (BirA) in the presence of 50 d-biotin in the LB medium and following induction with 2 mIPTG

The final His6-REX-TolA-AVI fusion proteins were produced as biotinylated proteins in the BL21 (DE3) BirA strain expressing biotin ligase (BirA) in the presence of 50 d-biotin in the LB medium and following induction with 2 mIPTG. for IL-23R binding in ELISA were confirmed to recognize human being IL-23R-IgG in surface plasmon resonance experiments, estimating the … Read more