[PMC free article] [PubMed] [Google Scholar] 18

[PMC free article] [PubMed] [Google Scholar] 18. assay reagents, such as dithionitrobenzene. Through the use of a combination of wild-type (WT) GlmU and a variant lacking acetyltransferase activity (GlmUH363N), we report here a robust coupled assay CENPA to screen for inhibitors specific to the acetyltransferase reaction of the GlmU enzyme. Using this assay, a total … Read more

Theoretical protein molecular weights were determined predicated on their amino acid solution sequences with no initial Met (http://workbench

Theoretical protein molecular weights were determined predicated on their amino acid solution sequences with no initial Met (http://workbench.sdsc.edu/) [25]. Crystallization of C274S and hDDAH-1 The very best quality DDAH-1 crystals were grown at 4 C by vapor diffusion using the sitting drop method from 0.1 M Tris-HCl pH 8.2, 20 % polyethylene glycol (PEG) 6000. … Read more