Cleared cell lysate was incubated with soluble CD81-GST fusion protein
Cleared cell lysate was incubated with soluble CD81-GST fusion protein. In addition to assaying infectivity, maker cell manifestation and HCVpp incorporation of HCV E2 proteins, CD81 binding profiles, and conformation of mutants were examined. Results Based on these characteristics, mutants either displayed wt characteristics (high infectivity [ 90% of wt HCVpp], CD81 binding, E1E2 manifestation, … Read more